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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLPS Polyclonal antibody (11766-1-AP)

    WB analysis using 11766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11766-1-AP (CLPS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using CLPS Polyclonal antibody (11766-1-AP)

    IHC staining of rat pancreas using 11766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using CLPS Polyclonal antibody (11766-1-AP)

    IHC staining of rat pancreas using 11766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CLPS Polyclonal antibody (11766-1-AP)

    IHC staining of mouse pancreas using 11766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CLPS Polyclonal antibody (11766-1-AP)

    IHC staining of mouse pancreas using 11766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using CLPS Polyclonal antibody (11766-1-AP)

    IF Staining of mouse pancreas using 11766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (11766-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CLPS Polyclonal antibody

CLPS Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 11766-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Colipase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inrat pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11766-1-AP targets CLPS in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLPS fusion protein Ag2321 相同性解析による交差性が予測される生物種
Full Name colipase, pancreatic
Calculated molecular weight 12 kDa
Observed molecular weight12 kDa
GenBank accession numberBC017897
Gene Symbol CLPS
Gene ID (NCBI) 1208
RRIDAB_10863117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

Protocols

Product Specific Protocols
WB protocol for CLPS antibody 11766-1-APDownload protocol
IHC protocol for CLPS antibody 11766-1-APDownload protocol
IF protocol for CLPS antibody 11766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols