Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
Product Information
15743-1-AP targets CLPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8165 Product name: Source: e coli.-derived, PET28a Tag: 6*His Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | ClpB caseinolytic peptidase B homolog (E. coli) |
| Calculated molecular weight | 707 aa, 79 kDa |
| Observed molecular weight | 58 kDa |
| GenBank accession number | BC006404 |
| Gene Symbol | CLPB |
| Gene ID (NCBI) | 81570 |
| RRID | AB_2847900 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H078 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CLPB encodes a protein of 707 aa with a predicted molecular mass of approximately 79 kDa, presenting two conserved ATP-binding domains. CLPB is a member of the AAA+ protein family. This potential internal translation initiation site suggests that a smaller form of ClpB, with a theoretical molecular mass of approximately 58 kDa, may be produced. (PMID: 25597510, PMID: 255957260)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLPB antibody 15743-1-AP | Download protocol |
| IHC protocol for CLPB antibody 15743-1-AP | Download protocol |
| IF protocol for CLPB antibody 15743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding | ||
Elife Skd3 (human ClpB) is a potent mitochondrial protein disaggregase that is inactivated by 3-methylglutaconic aciduria-linked mutations. | ||
iScience Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. | ||
Biochim Biophys Acta Mol Cell Res Impact of PARL-mediated mitochondrial protease activity on calcium regulation | ||
Elife Mitochondrial defects caused by PARL deficiency lead to arrested spermatogenesis and ferroptosis | ||
Int J Mol Sci Exploring Potential Diagnostic Biomarkers for Mechanical Asphyxia in the Heart Based on Proteomics Technology |