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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLN5 Recombinant antibody (85378-1-RR)

    WB analysis using 85378-1-RR (same clone as 85378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85378-1-RR (CLN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85378-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CLN5 Recombinant antibody (85378-1-RR)

    IF Staining of A431 using 85378-1-RR (same clone as 85378-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLN5 antibody (85378-1-RR, Clone: 242812H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85378-1-PBS in a different storage buffer formulation.

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CLN5 Recombinant antibody, PBS Only

CLN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85378-1-PBS
Clone No.242812H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Ceroid lipofuscinosis neuronal protein 5, BMPS, BMP synthase CLN5, Bis(monoacylglycero)phosphate synthase CLN5, secreted form, Bis(monoacylglycero)phosphate synthase CLN5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85378-1-PBS targets CLN5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLN5 fusion protein Ag18175 相同性解析による交差性が予測される生物種
Full Name ceroid-lipofuscinosis, neuronal 5
Calculated molecular weight 407 aa, 46 kDa
Observed molecular weight48 kDa
GenBank accession numberBC153154
Gene Symbol CLN5
Gene ID (NCBI) 1203
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75503
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The CLN5 (ceroid lipofuscinosis neuronal protein 5) protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. CLN5 plays a role in maintaining an acidic environment in the lysosomes, a critical feature for a functional lysosome (PMID: 38236309). Moreover, CLN5-deficient neuronal progenitor cells showed reduced thioesterase activity, confirming the live cell function of CLN5 in setting S-depalmitoylation levels (PMID: 35427157).