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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLN3 Monoclonal antibody (67957-1-Ig)

    WB analysis using 67957-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67957-1-Ig (CLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using CLN3 Monoclonal antibody (67957-1-Ig)

    WB analysis using 67957-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67957-1-Ig (CLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CLN3 Monoclonal antibody

CLN3 Monoclonal Antibody for WB, ELISA
Cat No. 67957-1-Ig
Clone No.1E10A9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

Batten disease protein, BATTENIN, BTS, CLN3, JNCL, Protein CLN3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NCCIT cells, NCI-H1299 cells, A549 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67957-1-Ig targets CLN3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag31402

Product name: Recombinant human CLN3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 300-438 aa of BC002394

Sequence: QGLFELLFFWNTSLSHAQQYRWYQMLYQAGVFASRSSLRCCRIRFTWALALLQCLNLVFLLADVWFGFLPSIYLVFLIILYEGLLGGAAYVNTFHNIALETSDEHREFAMAATCISDTLGISLSGLLALPLHDFLCQLS

相同性解析による交差性が予測される生物種
Full Name ceroid-lipofuscinosis, neuronal 3
Calculated molecular weight 438 aa, 48 kDa
Observed molecular weight50 kDa
GenBank accession numberBC002394
Gene Symbol CLN3
Gene ID (NCBI) 1201
RRIDAB_2918708
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane-spanning protein involved in the microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1 and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form a homodimeric complex (PMID: 10356317; 17286803).

Protocols

Product Specific Protocols
WB protocol for CLN3 antibody 67957-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols