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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLK3 Polyclonal antibody (28037-1-AP)

    WB analysis using 28037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28037-1-AP (CLK3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CLK3 Polyclonal antibody (28037-1-AP)

    IHC staining of human ovary cancer using 28037-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28037-1-AP (CLK3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CLK3 Polyclonal antibody

CLK3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28037-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.12.1, CDC-like kinase 3, CDC like kinase 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28037-1-AP targets CLK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLK3 fusion protein Ag27741 相同性解析による交差性が予測される生物種
Full Name CDC-like kinase 3
Calculated molecular weight 73 kDa
Observed molecular weight56 kDa
GenBank accession numberBC002555
Gene Symbol CLK3
Gene ID (NCBI) 1198
RRIDAB_3669638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49761
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLK3 (CDC Like Kinase 3) encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that functions on substrates containing serine/threonine and tyrosine. CLK3 modulates RNA splicing by phosphorylating serine/arginine-rich proteins such as SRSF1 and SRSF3. CLK3 dysregulation was indicated to be a high-penetrant factor in different types of human tumors even though its functions in tumors were not clearly characterized (PMID: 32453420). CLK3 has 4 isoforms, which is 74kDa, 59kDa, 56kDa and 18kDa.

Protocols

Product Specific Protocols
WB protocol for CLK3 antibody 28037-1-APDownload protocol
IHC protocol for CLK3 antibody 28037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols