Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse cerebellum tissue, rabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, A549 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67776-1-Ig targets CLIP-115/CLIP2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | Human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18902 Product name: Recombinant human CLIP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 671-1011 aa of BC150332 Sequence: QLEVQASQHRLELQEAQDQRRDAELRVHELEKLDVEYRGQAQAIEFLKEQISLAEKKMLDYERLQRAEAQGKQEVESLREKLLVAENRLQAVEALCSSQHTHMIESNDISEETIRTKETVEGLQDKLNKRDKEVTALTSQTEMLRAQVSALESKCKSGEKKVDALLKEKRRLEAELETVSRKTHDASGQLVLISQELLRKERSLNELRVLLLEANRHSPGPERDLSREVHKAEWRIKEQKLKDDIRGLREKLTGLDKEKSLSDQRRYSLIDPSSAPELLRLQHQLMSTEDALRDALDQAQQVEKLMEAMRSCPDKAQTIGNSGSANGIHQQDKAQKQEDKH 相同性解析による交差性が予測される生物種 |
| Full Name | CAP-GLY domain containing linker protein 2 |
| Calculated molecular weight | 1011 aa, 112 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC150332 |
| Gene Symbol | CLIP2 |
| Gene ID (NCBI) | 7461 |
| RRID | AB_2918541 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UDT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CLIP-115/CLIP2 antibody 67776-1-Ig | Download protocol |
| WB protocol for CLIP-115/CLIP2 antibody 67776-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |








