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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CLIC2 Recombinant antibody (83730-3-RR)

    WB analysis of K-562 using 83730-3-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83730-3-RR (CLIC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CLIC2 Recombinant antibody (83730-3-RR)

    WB analysis using 83730-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83730-3-RR (CLIC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83730-3-RR

    Affinity and Kinetic Characterization of 83730-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83730-3-RR against Human CLIC2 were performed. The affinity constant is below 1 pM.

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CLIC2 Recombinant antibody

CLIC2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83730-3-RR
Clone No.240726D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

XAP121, 240726D1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman testis tissue, K-562 cells, human heart tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83730-3-RR targets CLIC2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLIC2 fusion protein Ag6766 相同性解析による交差性が予測される生物種
Full Name chloride intracellular channel 2
Calculated molecular weight 28 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC005367
Gene Symbol CLIC2
Gene ID (NCBI) 1193
RRIDAB_3671329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15247
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLIC2 (chloride intracellular channel 2), also known as CLCNL2. It is expected to be located in cytoplasm and membrane. CLIC-2 exists as a monomer. It also has an unusual enzyme activity profile. While the CXXC active site motif is conserved between CLIC-2 and the glutaredoxins, no thiol transferase activity was detected. In contrast, low glutathione peroxidase activity was recorded. CLIC-2 was found to be widely distributed in tissues including heart and skeletal muscle. Functional studies showed that CLIC-2 inhibited cardiac ryanodine receptor Ca2+ release channels in lipid bilayers when added to the cytoplasmic side of the channels and inhibited Ca2+ release from cardiac sarcoplasmic reticulum vesicles. The calculated molecular weight of CLIC2 is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for CLIC2 antibody 83730-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols