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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CLEC9A Polyclonal antibody (55451-1-AP)

    IHC staining of human tonsillitis using 55451-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55451-1-AP (CLEC9A Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CLEC9A Polyclonal antibody (55451-1-AP)

    IHC staining of human tonsillitis using 55451-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55451-1-AP (CLEC9A Antibody) at dilution of 1:50 (under 40x lens).

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CLEC9A Polyclonal antibody

CLEC9A Polyclonal Antibody for IHC, ELISA
Cat No. 55451-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

UNQ9341/PRO34046, UNQ9341, DNGR1, C-type lectin domain family 9 member A, CD370

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55451-1-AP targets CLEC9A in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 9, member A
Calculated molecular weight 27 kDa
GenBank accession numberNM_207345
Gene Symbol CLEC9A
Gene ID (NCBI) 283420
RRIDAB_2881338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR1, CD370, is a type II membrane protein that contains C-type lectin domain. The C-type lectin domain family members share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. Mouse Clec9A is primarily restricted to dendritic cells (DCs) and selectively expressed in mouse CD8+ conventional DCs (cDCs) and plasmacytoid DCs (pDCs), whereas human CLEC9A is also expressed on human DC subtypes. CLEC9A functions as an activating receptor that recruits Syk kinase and can induce the production of proinflammatory cytokines. (PMID: 25553393; PMID: 15476922; PMID: 18669894; PMID: 18408006)

Protocols

Product Specific Protocols
IHC protocol for CLEC9A antibody 55451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols