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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLEC5A Recombinant monoclonal antibody (86633-2-RR)

    WB analysis using 86633-2-RR (same clone as 86633-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86633-2-RR (CLEC5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86633-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLEC5A Recombinant monoclonal antibody (86633-2-RR)

    WB analysis using 86633-2-RR (same clone as 86633-2-PBS)

    U-87 MG cells lysate was subjected to SDS PAGE followed by western blot with 86633-2-RR (CLEC5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86633-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86633-2-RR

    Affinity and Kinetic Characterization of 86633-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86633-2-RR against Human CLEC5A were performed. The affinity constant is 1.59 nM.

CLEC5A Recombinant monoclonal antibody, PBS Only

CLEC5A Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 86633-2-PBS
Clone No.251734A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CLECSF5, C-type lectin domain family 5 member A, C-type lectin superfamily member 5, MDL 1, MDL1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86633-2-PBS targets CLEC5A in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

DNA

相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 5, member A
Calculated molecular weight 188 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC112099
Gene Symbol CLEC5A
Gene ID (NCBI) 23601
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NY25
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C-type lectin domain family 5 member A (CLEC5A, also known as CLECSF5 and MDL1) is a spleen tyrosine kinase (Syk)-coupled receptor abundantly expressed by monocytes, macrophages and neutrophils (PMID: 10449773). It has a pivotal function in activating multiple aspects of innate immunity against bacterial invasion (PMID: 28824166). Both in vitro and in vivo evidence supported that CLEC5A was involved in glioblastoma pathogenesis via regulation of PI3K/Akt pathway (PMID: 30834619).

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