Validation Data Gallery
Tested Applications
| Positive WB detected in | IMR-32 cells, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34238-1-AP targets CLEC4F in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg7524 Product name: Recombinant Mouse CLEC4F protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 70-548 aa of / Sequence: RPEWNKEPPSLLLRGSNNSGHDNHSQFVRETEMQVAIQRLRDYEENSSSCHKEVQILKYQMDNVSSLVQLLGSHLEDVNADILQTKDVLKESGALALETQALRSSLEVASADIHSLRGDLEKANAMTSQTRGLLKSSTENTSAELHVLGRGLEEAQSEIQALRGSLQSANDLSSQTQGFLQHSMDNISAQIQTVRDGMERAGEKMNSLKKELETLTAQTQKANGHLEQTDAQIQGLKAELKSTSSLNSRIEVVNGQMKDASRELQTLRRDLSDVSALKSNVQMLQSNLQRAKTEMQTLKADLQATKALTAKIQGEQNRLGALQEAVAAQKQEQKTQNQVLQLIAQNWKYFNGNFYYFSRDKKPWREAEKFCTSQGAHLASVTSQEEQAFLVQTTSSGDHWIGLTDQGTEGIWRWVDGTPFNNAQSKGFWGKNQPDNWRHRNGEREDCVHVRQQWNDMACGSSYPWVCKKSTGWSAARVG 相同性解析による交差性が予測される生物種 |
| Full Name | C-type lectin domain family 4, member f |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | / |
| Gene Symbol | Clec4f |
| Gene ID (NCBI) | 51811 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P70194 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mouse CLEC4F (C-Type Lectin Domain Family 4 Member F) is a type II transmembrane glycoprotein primarily expressed on the surface of liver Kupffer cells, serving as a specific marker for liver-resident macrophages. As a pattern recognition receptor, CLEC4F recognizes and binds pathogen-associated sugar structures (such as fungal β-glucans and bacterial lipopolysaccharides) through its C-type lectin domain, mediating pathogen endocytosis and clearance, thereby playing a key role in the liver's innate immune defense.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC4F antibody 34238-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |