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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLEC18A Polyclonal antibody (21013-1-AP)

    WB analysis of HeLa using 21013-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21013-1-AP (CLEC18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CLEC18A Polyclonal antibody (21013-1-AP)

    WB analysis of HeLa using 21013-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21013-1-AP (CLEC18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLEC18A Polyclonal antibody (21013-1-AP)

    IHC staining of human kidney using 21013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLEC18A Polyclonal antibody (21013-1-AP)

    IHC staining of human kidney using 21013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CLEC18A Polyclonal antibody (21013-1-AP)

    IHC staining of human stomach using 21013-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CLEC18A Polyclonal antibody (21013-1-AP)

    IHC staining of human stomach using 21013-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 40x lens).

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CLEC18A Polyclonal antibody

CLEC18A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21013-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MRLP2, MRCL1, MRCL, Mannose receptor-like protein 2, C-type lectin domain family 18 member A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21013-1-AP targets CLEC18A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC18A fusion protein Ag15237 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 18, member A
Calculated molecular weight 446 aa, 50 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC141808
Gene Symbol CLEC18A
Gene ID (NCBI) 348174
RRIDAB_10732842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA5D8T8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC18A, or C-type lectin member 18A, is a protein that plays a significant role in the human immune system, particularly in the context of viral infections. CLEC18A is widely expressed, with the highest levels found in myeloid cells and the liver. It is a co-receptor for TLR3 (Toll-like receptor 3) and is involved in enhancing the host's immune response to viral infections, such as H5N1 influenza A virus (IAV). CLEC18A has been implicated in the development of mixed cryoglobulinemia (MC), an extrahepatic manifestation associated with hepatitis C virus (HCV). This antibody can recognize CLEC18A/B/C.

Protocols

Product Specific Protocols
WB protocol for CLEC18A antibody 21013-1-APDownload protocol
IHC protocol for CLEC18A antibody 21013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols