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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using CLEC14A Polyclonal antibody (23964-1-AP)

    WB analysis of human heart using 23964-1-AP (same clone as 23964-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23964-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CLEC14A Polyclonal antibody (23964-1-AP)

    WB analysis using 23964-1-AP (same clone as 23964-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23964-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CLEC14A Polyclonal antibody (23964-1-AP)

    WB analysis of mouse skeletal muscle using 23964-1-AP (same clone as 23964-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23964-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23964-1-PBS targets CLEC14A in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC14A fusion protein Ag21081 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 14, member A
Calculated molecular weight 490 aa, 52 kDa
Observed molecular weight 52 kDa, 100 kDa
GenBank accession numberBC031567
Gene Symbol CLEC14A
Gene ID (NCBI) 161198
RRIDAB_2879377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86T13
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).