• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLDN16 Recombinant antibody

CLDN16 Recombinant Antibody for IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230193G3

Cat no : 82954-1-RR

Synonyms

claudin 16, CLDN16, HOMG3, Paracellin 1, PCLN 1, PCLN1

Validation Data Gallery

Applications:
  • IF Staining of HEK-293 using 82954-1-RR

    IF Staining of HEK-293 using 82954-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-RR, Clone: 230193G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HEK-293 using 82954-1-RR

    FC experiment of HEK-293 using 82954-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human CLDN16 (82954-1-RR, Clone:230193G3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

82954-1-RR targets CLDN16 in IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLDN16 fusion protein Ag26531 相同性解析による交差性が予測される生物種
Full Name claudin 16
Calculated molecular weight 305 aa, 34 kDa
GenBank accession numberBC069682
Gene symbol CLDN16
Gene ID (NCBI) 10686
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CLDN16 antibody 82954-1-RRDownload protocol
FC protocol for CLDN16 antibody 82954-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols