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Validation Data Gallery

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  • Western Blot (WB) analysis of MDCK cells using CLCN5 Polyclonal antibody (26812-1-AP)

    WB analysis of MDCK using 26812-1-AP

    MDCK cells were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CLCN5 Polyclonal antibody (26812-1-AP)

    WB analysis of mouse brain using 26812-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26812-1-AP (CLCN5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLCN5 Polyclonal antibody (26812-1-AP)

    IHC staining of human kidney using 26812-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLCN5 Polyclonal antibody (26812-1-AP)

    IHC staining of human kidney using 26812-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26812-1-AP (CLCN5 Antibody) at dilution of 1:200 (under 40x lens).

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CLCN5 Polyclonal antibody

CLCN5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26812-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

chloride channel 5, Chloride channel protein 5, Chloride transporter ClC 5, ClC 5, CLC5, CLCK2, CLCN5, DENTS, hCIC K2, hClC K2, XLRH, XRN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDCK cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26812-1-AP targets CLCN5 in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine, mouse samples.

Tested Reactivity human, Canine, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCN5 fusion protein Ag25182 相同性解析による交差性が予測される生物種
Full Name chloride channel 5
Calculated molecular weight 83 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC130429
Gene Symbol CLCN5
Gene ID (NCBI) 1184
RRIDAB_2880643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51795
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CLCN5 antibody 26812-1-APDownload protocol
IHC protocol for CLCN5 antibody 26812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Gene Ther

Gene therapy of Dent disease type 1 in newborn ClC-5 null mice for sustained transgene expression and gene therapy effects

Authors - Pin Lyu
View Article