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Validation Data Gallery

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  • Western Blot (WB) analysis of rat heart tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of rat heart using 15346-1-AP (same clone as 15346-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of HEK-293 using 15346-1-AP (same clone as 15346-1-PBS)

    WB result of CKMT1A antibody (15346-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CKMT1A transfected HEK-293 cells. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of A549 cells using 15346-1-AP (same clone as 15346-1-PBS)

    A549 cells (shcontrol and shRNA of CKMT1A) were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:30. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of human colon using 15346-1-AP (same clone as 15346-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of human testis using 15346-1-AP (same clone as 15346-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of mouse heart using 15346-1-AP (same clone as 15346-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of mouse testis using 15346-1-AP (same clone as 15346-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    WB analysis of mouse testis using 15346-1-AP (same clone as 15346-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    IP experiment of mouse heart using 15346-1-AP (same clone as 15346-1-PBS)

    IP result of anti-CKMT1A (IP:15346-1-AP, 4ug; Detection:15346-1-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    IHC staining of human colon cancer using 15346-1-AP (same clone as 15346-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CKMT1A Polyclonal antibody (15346-1-AP)

    IHC staining of human breast cancer using 15346-1-AP (same clone as 15346-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15346-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CKMT1A Polyclonal antibody, PBS Only

CKMT1A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15346-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15346-1-PBS targets CKMT1A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7583

Product name: Recombinant human CKMT1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-417 aa of BC001926

Sequence: SERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFADLFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGGVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPTPVIHTKH

相同性解析による交差性が予測される生物種
Full Name creatine kinase, mitochondrial 1A
Calculated molecular weight 47 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC001926
Gene Symbol CKMT1A
Gene ID (NCBI) 548596
RRIDAB_2081073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP12532
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

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