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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKB Recombinant antibody (83522-5-RR)

    WB analysis using 83522-5-RR (same clone as 83522-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83522-5-RR (CKB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HAP1 cells using CKB Recombinant antibody (83522-5-RR)

    WB analysis of HAP1 using 83522-5-RR (same clone as 83522-5-PBS)

    WB result of sg-CKB antibody (83522-5-RR; 1:10000; room temperature for 1.5 hours) with wild-type and sg-CKB transfected HAP1 cells. This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00504-4

    Sandwich ELISA standard curve of MP00504-4

    Sandwich ELISA standard curve of MP00504-4, Human CKB Recombinant Matched Antibody Pair - PBS only. 83522-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7285. 83522-6-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.

  • Sample test of MP00504-4

    Sample test of MP00504-4

    The mean CKB concentration was determined to be 138.08 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 159.52 ng/mL in HeLa cell extract based on a 1.30 mg/mL extract load, 161.58 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load and 200.17 ng/mL in rat brain tissue extract based on a 8.20 mg/mL extract load.

  • Flow cytometry (FC) experiment of HeLa cells using CKB Recombinant antibody (83522-5-RR)

    FC experiment of HeLa using 83522-5-RR (same clone as 83522-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKB Recombinant Antibody (83522-5-RR, Clone:240418D2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83522-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83522-5-RR

    Affinity and Kinetic Characterization of 83522-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83522-5-RR against Human CKB were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

CKB Recombinant antibody, PBS Only (Capture)

CKB Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83522-5-PBS
Clone No.240418D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

KCRB, 240418D2, B CK, B-CK, CKBB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83522-5-PBS targets CKB as part of a matched antibody pair:

MP00504-4: 83522-5-PBS capture and 83522-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CKB fusion protein Ag7285 相同性解析による交差性が予測される生物種
Full Name creatine kinase, brain
Calculated molecular weight 43 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC001190
Gene Symbol CKB
Gene ID (NCBI) 1152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12277
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.