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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKAP4 Recombinant antibody (84485-4-RR)

    WB analysis using 84485-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84485-4-RR (CKAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CKAP4 Recombinant antibody (84485-4-RR)

    IF Staining of HepG2 using 84485-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (84485-4-RR, Clone: 241871B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CKAP4 Recombinant antibody (84485-4-RR)

    IF Staining of U2OS using 84485-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CKAP4 antibody (84485-4-RR, Clone: 241871B6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CKAP4 Recombinant antibody (84485-4-RR)

    FC experiment of HeLa using 84485-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CKAP4 Recombinant antibody (84485-4-RR, Clone:241871B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of U2OS cells using CKAP4 Recombinant antibody (84485-4-RR)

    FC experiment of U2OS using 84485-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug CKAP4 Recombinant antibody (84485-4-RR, Clone:241871B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84485-4-RR

    Affinity and Kinetic Characterization of 84485-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84485-4-RR against Human CKAP4 were performed. The affinity constant is 2.71 nM.

CKAP4 Recombinant monoclonal antibody

CKAP4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84485-4-RR
Clone No.241871B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241871B6, 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, A549 cells
Positive IF/ICC detected inHepG2 cells, U2OS cells
Positive FC (Intra) detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84485-4-RR targets CKAP4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10022

Product name: Recombinant human CKAP4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 254-602 aa of BC082972

Sequence: IFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV

相同性解析による交差性が予測される生物種
Full Name cytoskeleton-associated protein 4
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
RRIDAB_3672002
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ07065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).

Protocols

Product Specific Protocols
IF protocol for CKAP4 antibody 84485-4-RRDownload protocol
WB protocol for CKAP4 antibody 84485-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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