CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16686-1-AP

Synonyms

63 kDa membrane protein, CKAP4, CLIMP 63, ERGIC 63, p63



Tested Applications

Positive WB detected inA431 cells, rat kidney tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP4 fusion protein Ag10019 相同性解析による交差性が予測される生物種
Full Name cytoskeleton-associated protein 4
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC082972
Gene symbol CKAP4
Gene ID (NCBI) 10970
RRIDAB_2276275
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Protocols

Product Specific Protocols
WB protocol for CKAP4 antibody 16686-1-APDownload protocol
IHC protocol for CKAP4 antibody 16686-1-APDownload protocol
IF protocol for CKAP4 antibody 16686-1-APDownload protocol
IP protocol for CKAP4 antibody 16686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Regulation of endoplasmic reticulum turnover by selective autophagy.

Authors - Aliaksandr Khaminets
ratWB

Cell Metab

Palmitoylation couples insulin hypersecretion with β cell failure in diabetes

Authors - Guifang Dong
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex

Authors - Michael L van de Weijer
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Authors - Michael L van de Weijer
mouseIF

EMBO J

ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.

Authors - Ilaria Fregno
IF

EMBO J

MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.

Authors - Laura Cinque

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (06-29-2020)

Single strong band at correct size.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart