Validation Data Gallery
Tested Applications
| Positive WB detected in | NCI-H1299 cells, SGC-7901 cells, RAW 264.7 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86689-1-RR targets CKAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag21901 Product name: Recombinant human CKAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC136332 Sequence: MSTPAVPQDLQLPPSQRAQSAFKEQRRQKLKEHLLRRKTLFAYKQENEMLSSRDQRVVTSEDQVQEGTKVLKLKTKMADKENMKRPAESKNNTVVGKHCIPLKPSNELTNSTVVIDTHKPKDSNQTPHLLLTEDDPQSQHMTLSQAFHLKNNSKKKQMTTEKQKQDANMPKKPVLGSYRGQIVQSKINSFRKPLQVKDESSAATKKLSATIPKATKPQPVNTSSVTVKSNRSSNMTATTKFVSTTSQNTQLVRPPIRSHHSNTRDTVKQGISRTSANVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVVARPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRS 相同性解析による交差性が予測される生物種 |
| Full Name | cytoskeleton associated protein 2 |
| Calculated molecular weight | 683 aa, 77 kDa |
| Observed molecular weight | 75-85 kDa |
| GenBank accession number | BC136332 |
| Gene Symbol | CKAP2 |
| Gene ID (NCBI) | 26586 |
| RRID | AB_3745054 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WWK9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein (TMAP), is implicated in the regulation of cell proliferation (PMID: 30119212). CKAP2 is a MAP expressed during mitosis that localizes to centrosomes and spindles before being targeted for degradation by the anaphase-promoting complex/cyclosome at mitotic exit (PMID: 38381793, 17339342).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CKAP2 antibody 86689-1-RR | Download protocol |
| WB protocol for CKAP2 antibody 86689-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |