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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using CIT Polyclonal antibody (20033-1-AP)

    WB analysis of A431 using 20033-1-AP (same clone as 20033-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CIT Polyclonal antibody (20033-1-AP)

    WB analysis using 20033-1-AP (same clone as 20033-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CIT Polyclonal antibody (20033-1-AP)

    WB analysis of HeLa using 20033-1-AP (same clone as 20033-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CIT Polyclonal antibody (20033-1-AP)

    IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CIT Polyclonal antibody (20033-1-AP)

    IHC staining of mouse brain using 20033-1-AP (same clone as 20033-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20033-1-AP (CIT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CIT Polyclonal antibody (20033-1-AP)

    IF Staining of HepG2 using 20033-1-AP (same clone as 20033-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20033-1-PBS in a different storage buffer formulation.

CIT Polyclonal antibody, PBS Only

CIT Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20033-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20033-1-PBS targets CIT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name citron (rho-interacting, serine/threonine kinase 21)
Calculated molecular weight 231 kDa
Observed molecular weight 230 kDa, 177 kDa
GenBank accession numberNM_007174
Gene Symbol CIT
Gene ID (NCBI) 11113
RRIDAB_10665814
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14578
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.

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