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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CISD2 Recombinant antibody (82802-10-RR)

    WB analysis using 82802-10-RR (same clone as 82802-10-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82802-10-RR (CISD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CISD2 Recombinant antibody (82802-10-RR)

    IHC staining of mouse kidney using 82802-10-RR (same clone as 82802-10-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CISD2 Recombinant antibody (82802-10-RR)

    IHC staining of mouse kidney using 82802-10-RR (same clone as 82802-10-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CISD2 Recombinant antibody (82802-10-RR)

    IF Staining of HepG2 using 82802-10-RR (same clone as 82802-10-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (82802-10-RR, Clone: 6H13 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CISD2 Recombinant antibody (82802-10-RR)

    FC experiment of HepG2 using 82802-10-RR (same clone as 82802-10-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CISD2 Recombinant antibody (82802-10-RR, Clone:6H13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82802-10-RR

    Affinity and Kinetic Characterization of 82802-10-RR

    Biolayer interferometry (BLl) kinetic assays of 82802-10-RR against Human CISD2 were performed. The affinity constant is 3.07 nM.

CISD2 Recombinant monoclonal antibody, PBS Only

CISD2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82802-10-PBS
Clone No.6H13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2, CDGSH iron sulfur domain 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82802-10-PBS targets CISD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4172

Product name: Recombinant human CISD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-135 aa of BC032300

Sequence: PKKKQQKDSLINLKIQKENPKVVNEINIEDLCLTKAAYCRCWRSKTFPACDGSHNKHNELTGDNVGPLILKKKEV

相同性解析による交差性が予測される生物種
Full Name CDGSH iron sulfur domain 2
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC032300
Gene Symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8N5K1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

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