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  • Featured Product
  • KD/KO Validated

CISD2-Specific Monoclonal antibody

CISD2-Specific Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, zebrafish, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D7A3

Cat no : 66082-1-Ig

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, CISD2-Specific, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66082-1-Ig

    WB analysis of HeLa using 66082-1-Ig

    WB result of CISD2 antibody (66082-1-Ig, 1:3000) with si-Control and si-CISD2 transfected HeLa cells.

  • WB analysis of fetal human brain using 66082-1-Ig

    WB analysis of fetal human brain using 66082-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 66082-1-Ig

    WB analysis of MCF-7 using 66082-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of ROS1728 using 66082-1-Ig

    WB analysis of ROS1728 using 66082-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 66082-1-Ig

    WB analysis of RAW 264.7 using 66082-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of zebrafish using 66082-1-Ig

    WB analysis of zebrafish using 66082-1-Ig

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 66082-1-Ig (CISD2-Specific Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66082-1-Ig

    IHC staining of human kidney using 66082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 66082-1-Ig

    IHC staining of human kidney using 66082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 66082-1-Ig

    IHC staining of human testis using 66082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 66082-1-Ig

    IHC staining of human testis using 66082-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 66082-1-Ig

    IF Staining of MCF-7 using 66082-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66082-1-Ig(CISD2-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, MCF-7 cells, HeLa cells, ROS1728 cells, RAW 264.7 cells, zebrafish tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66082-1-Ig is CISD2-Specific Fusion Protein expressed in E. coli.

Tested Reactivity human, zebrafish, rat, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CDGSH iron sulfur domain 2
Observed molecular weight 15 kDa
GenBank accession numberNM_001008388
Gene symbol CISD2
Gene ID (NCBI) 493856
RRIDAB_11232425
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum. This monoclonal antibody is specific to CISD2 and does not cross-react with CISD1.

Protocols

Product Specific Protocols
WB protocol for CISD2-Specific antibody 66082-1-IgDownload protocol
IHC protocol for CISD2-Specific antibody 66082-1-IgDownload protocol
IF protocol for CISD2-Specific antibody 66082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biomed Sci

Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis

Authors - Yu-Wen Hung
View Article

Mol Neurodegener

Mitochondrial CISD1/Cisd accumulation blocks mitophagy and genetic or pharmacological inhibition rescues neurodegenerative phenotypes in Pink1/parkin models

Authors - Aitor Martinez
View Article