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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CIR Polyclonal antibody (32928-1-AP)

    WB analysis using 32928-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32928-1-AP (CIR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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CIR Polyclonal antibody

CIR Polyclonal Antibody for WB, ELISA
Cat No. 32928-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CBF1-interacting corepressor, CIR1, Corepressor interacting with RBPJ 1, Recepin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse liver tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32928-1-AP targets CIR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIR fusion protein Ag38647 相同性解析による交差性が予測される生物種
Full Name CBF1 interacting corepressor
Calculated molecular weight52kDa，450aa
Observed molecular weight23 kDa
GenBank accession numberNM_004882.3
Gene Symbol CIR
Gene ID (NCBI) 9541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86X95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor. CIR is a member of the family of SR-related proteins and that CIR plays a role in splicing regulation. In addition to a basic lysine and acidic serine-rich (BA) domain and a zinc knuckle-like motif, CIR has an arginine/serine dipeptide repeat (RS) domain in its C terminal region. The RS domain has been reported to be present in the superfamily of SR proteins, which are involved in splicing reactions (PMID: 15652350).

Protocols

Product Specific Protocols
WB protocol for CIR antibody 32928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols