Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
27600-1-AP targets CIDEB in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27003 Product name: Recombinant human CIDEB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 39-179 aa of BC035970 Sequence: RVCDHKRTIRKGLTAATRQELLAKALETLLLNGVLTLVLEEDGTAVDSEDFFQLLEDDTCLMVLQSGQSWSPTRSGVLSYGLGRERPKHSKDIARFTFDVYKQNPRDLFGSLNVKATFYGLYSMSCDFQGLGPKKVLRELL 相同性解析による交差性が予測される生物種 |
| Full Name | cell death-inducing DFFA-like effector b |
| Calculated molecular weight | 219 aa, 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC035970 |
| Gene Symbol | CIDEB |
| Gene ID (NCBI) | 27141 |
| RRID | AB_2880922 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cell death‐inducing DFFA‐like effector B (CIDEB), an ER and lipid droplet (LD)‐associated protein primarily expressed in the liver, participated in lipid metabolism by regulating lipid droplet fusion and VLDL lipidation. Although CIDEA is expressed at high levels in brown adipose tissue (BAT) and CIDEC is highly enriched in white adipose tissue (WAT) and BAT, CIDEB is more abundant in liver, kidney, small intestine, and colon. CIDEB is localized to endoplasmic reticulum and lipid droplets. (PMID: 30858281, PMID: 24831470, PMID: 19187774)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CIDEB antibody 27600-1-AP | Download protocol |
| WB protocol for CIDEB antibody 27600-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hazard Mater TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis. | ||
Cancer Biol Ther The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer | ||
Int J Mol Sci Unveiling Novel Mechanism of CIDEB in Fatty Acid Synthesis Through ChIP-Seq and Functional Analysis in Dairy Goat | ||
Mol Ther Nucleic Acids Targeting CIDEB alleviates liver steatosis and fibrosis in mouse MASH models |
