Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32585-1-AP targets CIB2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38508 Product name: Recombinant human CIB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of NM_006383.3 Sequence: MGNKQTIFTEEQLDNYQDCTFFNKKDILKLHSRFYELAPNLVPMDYRKSPIVHVPMSLIIQMPELRENPFKERIVAAFSEDGEGNLTFNDFVDMFSVLCESAPRELKANYAFKIYDFNTDNFICKEDLELTLARLTKSELDEEEVVLVCDKVIEEADLDGDGKLGFADFEDMIAKAPDFLSTFHIRI* 相同性解析による交差性が予測される生物種 |
| Full Name | calcium and integrin binding family member 2 |
| Calculated molecular weight | 22 kDa，187aa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | NM_006383.3 |
| Gene Symbol | CIB2 |
| Gene ID (NCBI) | 10518 |
| RRID | AB_3742663 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O75838 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The CIB (calcium and integrin binding) protein family has 4 members (CIB1-CIB4), each composed of 4 EF-hand Ca2+-binding motifs (PMID: 27771768). CIB2 is broadly expressed in a variety of tissues, which plays a role in sensory neurons of the ear and eye involved in the regulation of Ca2+ homeostasis (PMID: 28663585; 29084757; 29255404). Mutations of CIB2 have been associated with autosomal recessive nonsyndromic deafness 48 (DFNB48), as well as syndromic deafness in Usher syndrome, type 1J (USH1J) (PMID: 23331261). The observed molecular weight of CIB2 is 39 kDa because it can form a dimer (PMID: 30174586).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CIB2 antibody 32585-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |