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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using CIB1 Polyclonal antibody (11823-1-AP)

    WB analysis of human liver using 11823-1-AP (same clone as 11823-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CIB1 Polyclonal antibody (11823-1-AP)

    WB analysis of HepG2 using 11823-1-AP (same clone as 11823-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using CIB1 Polyclonal antibody (11823-1-AP)

    WB analysis of rat testis using 11823-1-AP (same clone as 11823-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 11823-1-AP (CIB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using CIB1 Polyclonal antibody (11823-1-AP)

    IP experiment of mouse testis using 11823-1-AP (same clone as 11823-1-PBS)

    IP result of anti-CIB1 (IP:11823-1-AP, 4ug; Detection:11823-1-AP 1:300) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CIB1 Polyclonal antibody (11823-1-AP)

    IHC staining of human breast cancer using 11823-1-AP (same clone as 11823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CIB1 Polyclonal antibody (11823-1-AP)

    IHC staining of human testis using 11823-1-AP (same clone as 11823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CIB1 Polyclonal antibody (11823-1-AP)

    IHC staining of human testis using 11823-1-AP (same clone as 11823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CIB1 Polyclonal antibody (11823-1-AP)

    IHC staining of rat testis using 11823-1-AP (same clone as 11823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CIB1 Polyclonal antibody (11823-1-AP)

    IHC staining of rat testis using 11823-1-AP (same clone as 11823-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis using 11823-1-AP (CIB1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CIB1 Polyclonal antibody (11823-1-AP)

    IF Staining of A549 using 11823-1-AP (same clone as 11823-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CIB1 antibody (11823-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11823-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CIB1 Polyclonal antibody, PBS Only

CIB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11823-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11823-1-PBS targets CIB1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2391

Product name: Recombinant human CIB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-191 aa of BC000846

Sequence: MGGSGSRLSKELLAEYQDLTFLTKQEILLAHRRFCELLPQEQRSVESSLRAQVPFEQILSLPELKANPFKERICRVFSTSPAKDSLSFEDFLDLLSVFSDTATPDIKSHYAFRIFDFDDDGTLNREDLSRLVNCLTGEGEDTRLSASEMKQLIDNILEESDIDRDGTINLSEFQHVISRSPDFASSFKIVL

相同性解析による交差性が予測される生物種
Full Name calcium and integrin binding 1 (calmyrin)
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC000846
Gene Symbol CIB1
Gene ID (NCBI) 10519
RRIDAB_2079679
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99828
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.

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