Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24444-1-AP targets CHSY3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19641 Product name: Recombinant human CHSY3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 571-769 aa of BC137325 Sequence: FFRETEELDVNSLVESINSETQSFSFISNSLKILSSFQGAKEMGGHNEKKVHILVPLIGRYDIFLRFMENFENMCLIPKQNVKLVIILFSRDSGQDSSKHIELIKGYQNKYPKAEMTLIPMKGEFSRGLGLEMASAQFDNDTLLLFCDVDLIFREDFLQRCRDNTIQGQQVYYPIIFSQYDPKVTNGGNPPTDDYFIFS 相同性解析による交差性が予測される生物種 |
| Full Name | chondroitin sulfate synthase 3 |
| Calculated molecular weight | 882 aa, 100 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC137325 |
| Gene Symbol | CHSY3 |
| Gene ID (NCBI) | 337876 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70JA7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHSY3 is a Golgi-localized chondroitin sulfate synthase that controls glycosaminoglycan chain elongation on extracellular matrix proteoglycans. By regulating chondroitin sulfate structure, CHSY3 influences matrix organization, growth factor signaling, and tissue mechanics. Its dysregulation is implicated in cartilage pathology, pulmonary remodeling, and cancer progression, highlighting CHSY3 as a key enzymatic regulator of extracellular matrix biology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHSY3 antibody 24444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |