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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHRNA9 Recombinant monoclonal antibody (87585-1-RR)

    WB analysis using 87585-1-RR (same clone as 87585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87585-1-RR (CHRNA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CHRNA9 Recombinant monoclonal antibody (87585-1-RR)

    WB analysis of HeLa using 87585-1-RR (same clone as 87585-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 87585-1-RR (CHRNA9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87585-1-PBS in a different storage buffer formulation.

CHRNA9 Recombinant monoclonal antibody, PBS Only

CHRNA9 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87585-1-PBS
Clone No.252931C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

HSA243342, NACHR alpha 9, NACHR alpha-9, NACHRA9, Neuronal acetylcholine receptor subunit alpha-9

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87585-1-PBS targets CHRNA9 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag23272

Product name: Recombinant human CHRNA9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 324-479 aa of BC113549

Sequence: HFCGAEARPVPHWARVVILKYMSRVLFVYDVGESCLSPHHSRERDHLTKVYSKLPESNLKAARNKDLSRKKDMNKRLKNDLGCQGKNPQEAESYCAQYKVLTRNIEYIAKCLKDHKATSSKGSEWKKVAKVIDRFFMWIFFIMVFVMTILIIARAD

相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 9
Calculated molecular weight 479 aa, 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC113549
Gene Symbol CHRNA9
Gene ID (NCBI) 55584
RRIDAB_3745638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UGM1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHRNA9 (nicotinic α9 subunit of cholinergic receptor) gene is located in zone 4 (4p14) of short arm 1 of human chromosome 4, encoding a ligand-gated divalent cation channel protein, belonging to the nicotinic acetylcholine receptor (nAChR) superfamily. This subunit can be combined with CHRNA10 subunit to form a heteropentamer or a homopentamer, which is mainly distributed in cochlear outer hair cells, dorsal root ganglion, keratinocytes and immune cells.

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