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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHRNA7 Recombinant antibody (82848-4-RR)

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CHRNA7 Recombinant antibody (82848-4-RR)

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CHRNA7 Recombinant antibody (82848-4-RR)

    FC experiment of SH-SY5Y using 82848-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of Jurkat cells using CHRNA7 Recombinant antibody (82848-4-RR)

    FC experiment of Jurkat using 82848-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 82848-4-RR

    Affinity and Kinetic Characterization of 82848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is below 1 pM.

CHRNA7 Recombinant monoclonal antibody

CHRNA7 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82848-4-RR
Clone No.240117D12
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

240117D12, CHRNA7 2, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82848-4-RR targets CHRNA7 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15980

Product name: Recombinant human CHRNA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 130-321 aa of BC037571

Sequence: TVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA

相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 7
Calculated molecular weight 502 aa, 56 kDa
Observed molecular weight50 kDa, 56 kDa
GenBank accession numberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
RRIDAB_3670575
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP36544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

Protocols

Product Specific Protocols
FC protocol for CHRNA7 antibody 82848-4-RRDownload protocol
WB protocol for CHRNA7 antibody 82848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Neuromolecular Med

Hypertension Induces Cognitive Impairment and Alterations of Acetylcholine Receptor mRNA Expression in the Hippocampus and Cortex of Mice

Authors - Jingfeng Liu
View Article
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