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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using CHPF N-terminal Polyclonal antibody (23953-1-AP)

    WB analysis of human placenta using 23953-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CHPF N-terminal Polyclonal antibody (23953-1-AP)

    WB analysis of mouse skeletal muscle using 23953-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using CHPF N-terminal Polyclonal antibody (23953-1-AP)

    WB analysis of C6 using 23953-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 23953-1-AP (CHPF N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CHPF N-terminal Polyclonal antibody (23953-1-AP)

    IHC staining of mouse heart using 23953-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CHPF N-terminal Polyclonal antibody (23953-1-AP)

    IHC staining of mouse heart using 23953-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23953-1-AP (CHPF N-terminal antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHPF N-terminal Polyclonal antibody (23953-1-AP)

    IF Staining of HepG2 using 23953-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHPF N-terminal antibody (23953-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

CHPF N-terminal Polyclonal antibody

CHPF N-terminal Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 23953-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ChPF, Chondroitin-polymerizing factor, EC:2.4.1.175, CSS2, CHSY2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse skeletal muscle tissue, C6 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23953-1-AP targets CHPF N-terminal in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHPF N-terminal fusion protein Ag20819 相同性解析による交差性が予測される生物種
Full Name chondroitin polymerizing factor
Calculated molecular weight 85 kDa
Observed molecular weight 85-90 kDa, 65-70 kDa
GenBank accession numberBC023531
Gene Symbol CHPF
Gene ID (NCBI) 79586
RRIDAB_2879373
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8IZ52
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHPF N-terminal antibody 23953-1-APDownload protocol
IHC protocol for CHPF N-terminal antibody 23953-1-APDownload protocol
IF protocol for CHPF N-terminal antibody 23953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

Targeting Chondroitin Sulfate Reduces Invasiveness of Glioma Cells by Suppressing CD44 and Integrin β1 Expression.

Authors - Yin-Hung Chu
View Article
humanIHC,WB

J Cell Commun Signal

Chondroitin polymerizing factor predicts a poor prognosis and promotes breast cancer progression via the upstream TGF-β1/SMAD3 and JNK axis activation

Authors - Qiang-Feng Pan
View Article
humanWB,IHC,IF

Aging (Albany NY)

The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma

Authors - Haitao Luo
  • KD Validated
View Article