CHMP6 Polyclonal antibody

Name: CHMP6 Polyclonal antibody
Brand: Proteintech
SKU: 16278-1-AP

CHMP6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16278-1-AP

Synonyms

CHMP6, chromatin modifying protein 6, hVps20, VPS20

Validation Data Gallery

Applications:

AllWBIP

WB analysis of mouse testis using 16278-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16278-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 16278-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 16278-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 16278-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 16278-1-AP (CHMP6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 16278-1-AP
IP result of anti-CHMP6 (IP:16278-1-AP, 4ug; Detection:16278-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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Cat No. 16278-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, A375 cells, A431 cells, Jurkat cells, U-937 cells
Positive IP detected in	mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below

Product Information

16278-1-AP targets CHMP6 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CHMP6 fusion protein Ag9357 相同性解析による交差性が予測される生物種
Full Name	chromatin modifying protein 6
Calculated molecular weight	201 aa, 23 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC010108
Gene symbol	CHMP6
Gene ID (NCBI)	79643
RRID	AB_2079498
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CHMP6 antibody 16278-1-AP	Download protocol
IP protocol for CHMP6 antibody 16278-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. Authors - Vincent Mercier View Article
canine	WB	Curr Biol A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission. Authors - Eva P Karasmanis View Article
human	WB	J Virol A non-envelopment role for the ESCRT-III complex during HCMV infection. Authors - Nicholas T Streck View Article
human	WB	J Virol Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread. Authors - Nicholas T Streck View Article
human	WB,IF	Nat Commun Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy Authors - Takashi Nozawa KD Validated View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHMP6 Polyclonal antibody

CHMP6 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis of mouse testis using 16278-1-AP

WB analysis of Jurkat using 16278-1-AP

WB analysis of U-937 using 16278-1-AP

WB analysis of A375 using 16278-1-AP

WB analysis of A431 using 16278-1-AP

IP experiment of mouse testis using 16278-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

A non-envelopment role for the ESCRT-III complex during HCMV infection.

Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.

Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy