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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4C Recombinant monoclonal antibody (87800-1-RR)

    WB analysis using 87800-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87800-1-RR (CHMP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Human lung cancer （Adenocarcinoma） using CHMP4C Recombinant monoclonal antibody (87800-1-RR)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87800-1-RR

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87800-1-RR (CHMP4C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of Human lung cancer （Adenocarcinoma） using CHMP4C Recombinant monoclonal antibody (87800-1-RR)

    IHC staining of Human lung cancer （Adenocarcinoma） using 87800-1-RR

    Immunohistochemical analysis of paraffin-embedded Human lung cancer (Adenocarcinoma) slide using 87800-1-RR (CHMP4C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHMP4C Recombinant monoclonal antibody

CHMP4C Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87800-1-RR
Clone No.251884A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

chromatin modifying protein 4C, Chromatin-modifying protein 4c, hSnf7 3, hSnf7-3, hVps32 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, NCI-H1299 cells, A549 cells, LNCap cells, SiHa cells, TT cells
Positive IHC detected inHuman lung cancer （Adenocarcinoma）
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87800-1-RR targets CHMP4C in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9577

Product name: Recombinant human CHMP4C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-233 aa of BC014321

Sequence: MSKLGKFFKGGGSSKSRAAPSPQEALVRLRETEEMLGKKQEYLENRIQREIALAKKHGTQNKRAALQALKRKKRFEKQLTQIDGTLSTIEFQREALENSHTNTEVLRNMGFAAKAMKSVHENMDLNKIDDLMQEITEQQDIAQEISEAFSQRVGFGDDFDEDELMAELEELEQEELNKKMTNIRLPNVPSSSLPAQPNRKPGMSSTARRSRAASSQRAEEEDDDIKQLAAWAT

相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 4C
Calculated molecular weight 233 aa, 26 kDa
Observed molecular weight35 -42 kDa
GenBank accession numberBC014321
Gene Symbol CHMP4C
Gene ID (NCBI) 92421
RRIDAB_3745758
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96CF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHMP4C (Charged multivesicular body protein 4c), also known as SHAX3. It is expected to be located in the cytoplasm and membrane, which is expressed in heart, spleen and kidney. CHMP4C is one of the charged multivesicular protein (CHMP), and is involved in the composition of the endosomal sorting complex required for transport III (ESCRT-III), facilitating the necessary separation of daughter cells. CHMP4C has been proposed to be involved in the progression of different carcinomas. The molecular weight of CHMP4C is 26 kDa. Phosphorylated at Ser-210 by AURKB during cytokinesis: together with ZFYVE19/ANCHR, phosphorylated CHMP4C retains abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Phosphorylation and ubiquitination modification may lead to molecular weight of 35-42kd.

Protocols

Product Specific Protocols
IHC protocol for CHMP4C antibody 87800-1-RRDownload protocol
WB protocol for CHMP4C antibody 87800-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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