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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHMP4B Polyclonal antibody (13683-1-AP)

    WB analysis using 13683-1-AP (same clone as 13683-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using CHMP4B Polyclonal antibody (13683-1-AP)

    WB analysis of mouse heart using 13683-1-AP (same clone as 13683-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CHMP4B Polyclonal antibody (13683-1-AP)

    WB analysis of HeLa using 13683-1-AP (same clone as 13683-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using CHMP4B Polyclonal antibody (13683-1-AP)

    WB analysis of rat heart using 13683-1-AP (same clone as 13683-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CHMP4B Polyclonal antibody (13683-1-AP)

    IF Staining of C2C12 using 13683-1-AP (same clone as 13683-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using CHMP4B Polyclonal antibody (13683-1-AP)

    IF Staining of H9C2 using 13683-1-AP (same clone as 13683-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CHMP4B Polyclonal antibody (13683-1-AP)

    IF Staining of C2C12 using 13683-1-AP (same clone as 13683-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13683-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHMP4B Polyclonal antibody, PBS Only

CHMP4B Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13683-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C20orf178, chromatin modifying protein 4B, Chromatin-modifying protein 4b, hSnf7-2, hVps32-2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13683-1-PBS targets CHMP4B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4544

Product name: Recombinant human CHMP4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC033859

Sequence: MSVFGKLFGAGGGKAGKGGPTPQEAIQRLRDTEEMLSKKQEFLEKKIEQELTAAKKHGTKNKRAALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGYAAKAMKAAHDNMDIDKVDELMQDIADQQELAEEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLPNVPSIALPSKPAKKKEEEDDDMKELENWAGSM

相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 4B
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight25-33 kDa
GenBank accession numberBC033859
Gene Symbol CHMP4B
Gene ID (NCBI) 128866
RRIDAB_2877971
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H444
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

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