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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human heart using 12527-1-AP (same clone as 12527-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human ileum tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human ileum using 12527-1-AP (same clone as 12527-1-PBS)

    human ileum tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human kidney using 12527-1-AP (same clone as 12527-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human placenta using 12527-1-AP (same clone as 12527-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    WB analysis of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver cancer using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver cancer using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CHMP2B Polyclonal antibody (12527-1-AP)

    IHC staining of human liver using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 12527-1-AP (CHMP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHMP2B Polyclonal antibody (12527-1-AP)

    IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PFA fixed cells using CHMP2B Polyclonal antibody (12527-1-AP)

    IF Staining of PFA fixed cells using 12527-1-AP (same clone as 12527-1-PBS)

    IF result (endosomal stain) of anti-CHMP2B (12527-1-AP) with the cell (PFA fixed) which has been treated with siRNA for VPS4 by Dr. Philip Woodman. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHMP2B Polyclonal antibody (12527-1-AP)

    IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CHMP2B antibody 12527-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHMP2B Polyclonal antibody, PBS Only

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12527-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CGI-84, CHMP2.5, chromatin modifying protein 2B, Chromatin-modifying protein 2b, DMT1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12527-1-PBS targets CHMP2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3222

Product name: Recombinant human CHMP2B protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-213 aa of BC001553

Sequence: MASLFKKKTVDDVIKEQNRELRGTQRAIIRDRAALEKQEKQLELEIKKMAKIGNKEACKVLAKQLVHLRKQKTRTFAVSSKVTSMSTQTKVMNSQMKMAGAMSTTAKTMQAVNKKMDPQKTLQTMQNFQKENMKMEMTEEMINDTLDDIFDGSDDEEESQDIVNQVLDEIGIEISGKMAKAPSAARSLPSASTSKATISDEEIERQLKALGVD

相同性解析による交差性が予測される生物種
Full Name chromatin modifying protein 2B
Calculated molecular weight 24 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001553
Gene Symbol CHMP2B
Gene ID (NCBI) 25978
RRIDAB_10603358
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQN3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.

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