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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

CHL1 Polyclonal antibody

CHL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25250-1-AP

Synonyms

CALL, CHL1, Close homolog of L1, L1CAM2

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 25250-1-AP

    WB analysis of mouse brain using 25250-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25250-1-AP (CHL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COS-7 using 25250-1-AP

    WB analysis of COS-7 using 25250-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 25250-1-AP (CHL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, COS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25250-1-AP is CHL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, monkey
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHL1 fusion protein Ag19263 相同性解析による交差性が予測される生物種
Full Name cell adhesion molecule with homology to L1CAM (close homolog of L1)
Calculated molecular weight 1208 aa, 135 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC104918
Gene symbol CHL1
Gene ID (NCBI) 10752
RRIDAB_2879988
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHL1 antibody 25250-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Transl Med

Tau and other proteins found in Alzheimer's disease spinal fluid are linked to retromer-mediated endosomal traffic in mice and humans.

Authors - Sabrina Simoes
View Article
humanWB

Oncol Lett

Overexpression of close homolog of L1 enhances the chemosensitivity of lung cancer cells via inhibition of the Akt pathway.

Authors - Xiangdao Cai
  • KD Validated
View Article