Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13520-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline Kinase Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse colon using 13520-1-AP
mouse colon tissue were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 13520-1-AP
IP result of anti-Choline Kinase Alpha (IP:13520-1-AP, 4ug; Detection:13520-1-AP 1:500) with mouse spleen tissue lysate 4000ug.
IHC staining of human liver cancer using 13520-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver cancer using 13520-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 13520-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Choline Kinase Alpha antibody (13520-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of HeLa using 13520-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13520-1-AP (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Choline Kinase Alpha Polyclonal antibody

Choline Kinase Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13520-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CHKA, CHETK-alpha, CHK, CKI, EC:2.7.1.32

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (24)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, mouse colon tissue, mouse spleen tissue, rat spleen tissue
Positive IP detected in	mouse spleen tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 20 publications below
IHC	See 6 publications below
IF	See 2 publications below
ELISA	See 1 publications below

Product Information

13520-1-AP targets Choline Kinase Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4761 Product name: Recombinant human CHKA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-439 aa of BC036471 Sequence: EPRKVLLRLYGAILQVGAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELGLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGQWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV 相同性解析による交差性が予測される生物種
Full Name	choline kinase alpha
Calculated molecular weight	439 aa, 52 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC036471
Gene Symbol	CHKA
Gene ID (NCBI)	1119
RRID	AB_2079160
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35790
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.

Protocols

Product Specific Protocols
IF protocol for Choline Kinase Alpha antibody 13520-1-AP	Download protocol
IHC protocol for Choline Kinase Alpha antibody 13520-1-AP	Download protocol
IP protocol for Choline Kinase Alpha antibody 13520-1-AP	Download protocol
WB protocol for Choline Kinase Alpha antibody 13520-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation Authors - Qingxia Ma View Article
mouse	WB,IHC	Ann Rheum Dis Choline kinase inhibition in rheumatoid arthritis. Authors - M Guma View Article
human	WB	Clin Cancer Res Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment. Authors - Roopali Saxena View Article
human	IHC	Cell Mol Life Sci Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling Authors - Kunxiang Gong View Article
mouse	WB	Neurobiol Dis Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis Authors - Fan Zhang View Article
mouse	WB	Front Cell Dev Biol Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration. Authors - Ming Kong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13520-1-AP

WB analysis of mouse colon using 13520-1-AP

IP experiment of mouse spleen using 13520-1-AP

IHC staining of human liver cancer using 13520-1-AP

IHC staining of human liver cancer using 13520-1-AP

IF Staining of HeLa using 13520-1-AP

IF Staining of HeLa using 13520-1-AP

Choline Kinase Alpha Polyclonal antibody

Choline Kinase Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation

Choline kinase inhibition in rheumatoid arthritis.

Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment.

Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling

Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis

Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.