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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    WB analysis of mouse lung using 21484-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    WB analysis of mouse lung using 21484-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human stomach using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human stomach using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human lung using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using CHIA Polyclonal antibody (21484-1-AP)

    IHC staining of human lung using 21484-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHIA Polyclonal antibody (21484-1-AP)

    IF Staining of HepG2 using 21484-1-AP

    Immunofluorescent analysis of HepG2 cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHIA Polyclonal antibody (21484-1-AP)

    IF Staining of HeLa using 21484-1-AP

    Immunofluorescent analysis of Hela cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CHIA Polyclonal antibody

CHIA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21484-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TSA1902, Lung-specific protein TSA1902, Lung specific protein TSA1902, EC:3.2.1.14, AMCase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman stomach tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21484-1-AP targets CHIA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15767

Product name: Recombinant human CHIA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC036339

Sequence: MVSTPENRQTFITSVIKFLRQYEFDGLDFDWEYPGSRGSPPQDKHLFTVLVQEMREAFEQEAKQINKPRLMVTAAVAAGISNIQSGYEIPQLSQYLDYIHVMTYDLHGSWEGYTGENSPLYKYPTDTGSNAYLNVDYVMNYWKDNGAPAEKLIVGFPTYGHNFILSNPSNTGIGAPTSGAGPAGPYAKESGIWAYYEICTFLKNGATQGWDAPQEVPYAYQGNVWVGYDNIKSFDIKAQWLKHNKFGGAMVWAIDLDDFTGTFCNQGKFPLISTLKKALGLQSASCTAPAQPIEPITAAPSGSGNGSGSSSSGGSSGGSGFCAVRANGLYPVANNRNAFWHCVNGVTYQQNCQAGLVFDTSCDCCNWA

相同性解析による交差性が予測される生物種
Full Name chitinase, acidic
Calculated molecular weight 476 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC036339
Gene Symbol CHIA
Gene ID (NCBI) 27159
RRIDAB_10755301
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZP6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CHIA antibody 21484-1-APDownload protocol
IHC protocol for CHIA antibody 21484-1-APDownload protocol
WB protocol for CHIA antibody 21484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis.

Authors - Lei Zhao
View Article
mouseIF

Commun Biol

Elevated stress response marks deeply quiescent reserve cells of gastric chief cells

Authors - Daisuke Shiokawa
View Article
mouseWB,IF

Eur J Immunol

Aspergillus fumigatus corneal infection is regulated by chitin synthases and by neutrophil-derived acidic mammalian chitinase.

Authors - Steven de Jesus Carrion
View Article
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