• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHI3L1 Polyclonal antibody

CHI3L1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30419-1-AP

Synonyms

39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40

Validation Data Gallery

Applications:
  • WB analysis using 30419-1-AP

    WB analysis using 30419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30419-1-AP (CHI3L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 30419-1-AP

    IF Staining of mouse brain using 30419-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1 antibody (30419-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHT-29 cells, HepG2 cells
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30419-1-AP targets CHI3L1 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHI3L1 fusion protein Ag33123 相同性解析による交差性が予測される生物種
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Calculated molecular weight 383 aa, 43 kDa
Observed molecular weight40-43 kDa
GenBank accession numberBC008568
Gene symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHI3L1 antibody 30419-1-APDownload protocol
IF protocol for CHI3L1 antibody 30419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols