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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHGB Recombinant antibody (85438-4-RR)

    WB analysis using 85438-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CHGB Recombinant antibody (85438-4-RR)

    WB analysis using 85438-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85438-4-RR

    Affinity and Kinetic Characterization of 85438-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85438-4-RR against Human CHGB were performed. The affinity constant is 1.95 nM.

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CHGB Recombinant antibody

CHGB Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85438-4-RR
Clone No.242952C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CgB, Chromogranin B, SCG1, 242952C9, Chromogranin-B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, BxPC-3 cells, SH-SY5Y cells, RAW 264.7 cells, PC-12 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85438-4-RR targets CHGB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHGB fusion protein Ag6833 相同性解析による交差性が予測される生物種
Full Name chromogranin B (secretogranin 1)
Calculated molecular weight 78 kDa
Observed molecular weight 70-100 kDa
GenBank accession numberBC000375
Gene Symbol Chromogranin B
Gene ID (NCBI) 1114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05060
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa.

Protocols

Product Specific Protocols
WB protocol for CHGB antibody 85438-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols