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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Chromogranin A Recombinant antibody (85605-2-RR)

    WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Chromogranin A Recombinant antibody (85605-2-RR)

    WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

    TT cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Chromogranin A Recombinant antibody (85605-2-RR)

    WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Chromogranin A Recombinant antibody (85605-2-RR)

    WB analysis using 85605-2-RR (same clone as 85605-2-PBS)

    Pig adrenal gland tissue was subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IHC staining of mouse pancreas using 85605-2-RR (same clone as 85605-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendix tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IHC staining of human appendix using 85605-2-RR (same clone as 85605-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IHC staining of mouse colon using 85605-2-RR (same clone as 85605-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IHC staining of human colon using 85605-2-RR (same clone as 85605-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IHC staining of human pancreas using 85605-2-RR (same clone as 85605-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IHC staining of human pancreas using 85605-2-RR (same clone as 85605-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IF Staining of mouse pancreas using 85605-2-RR (same clone as 85605-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using Chromogranin A Recombinant antibody (85605-2-RR)

    IF Staining of mouse small intestine using 85605-2-RR (same clone as 85605-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85605-2-PBS in a different storage buffer formulation.

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Chromogranin A Recombinant antibody, PBS Only

Chromogranin A Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85605-2-PBS
Clone No.243048H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CGA, CHGA, AL-11, Chromogranin-A, EA-92

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85605-2-PBS targets Chromogranin A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Chromogranin A fusion protein Ag19780 相同性解析による交差性が予測される生物種
Full Name chromogranin A (parathyroid secretory protein 1)
Calculated molecular weight 51 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC006459
Gene Symbol Chromogranin A
Gene ID (NCBI) 1113
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10645
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.