Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, multi-cells/tissue |
| Positive IHC detected in | human endometrial cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
Product Information
12169-1-AP targets CHFR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2811 Product name: Recombinant human CHFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 344-652 aa of BC012072 Sequence: NNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN 相同性解析による交差性が予測される生物種 |
| Full Name | checkpoint with forkhead and ring finger domains |
| Calculated molecular weight | 664 aa, 73 kDa |
| Observed molecular weight | 70-85 kDa |
| GenBank accession number | BC012072 |
| Gene Symbol | CHFR |
| Gene ID (NCBI) | 55743 |
| RRID | AB_2080821 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Checkpoint with forkhead and RING finger domains protein(CHFR) is a checkpoint protein that reportedly initiates a cell cycle delay in response to microtubule stress during prophase in mitosis and it has been hypothesized to be a tumor suppressor with a potential role as a biomarker for chemotherapeutic response to Taxol(PMID:17596595). It belongs to the CHFR family. Endogenous Chfr is ubiquitinated and phosphorylated in an MPF-dependent manner in mitotic Xenopus egg extracts(PMID:18504434). It has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CHFR antibody 12169-1-AP | Download protocol |
| WB protocol for CHFR antibody 12169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions
| ||
Clin Cancer Res Association of CHFR promoter methylation with disease recurrence in locally advanced colon cancer. | ||
Mol Med Rep CHFR‑mediated epithelial‑to‑mesenchymal transition promotes metastasis in human breast cancer cells.
| ||
J Biol Chem CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation. | ||
Front Genet Identification of a Novel Epigenetic Signature CHFR as a Potential Prognostic Gene Involved in Metastatic Clear Cell Renal Cell Carcinoma.
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