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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHDH Polyclonal antibody (17356-1-AP)

    WB analysis using 17356-1-AP (same clone as 17356-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CHDH Polyclonal antibody (17356-1-AP)

    WB analysis of mouse kidney using 17356-1-AP (same clone as 17356-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CHDH Polyclonal antibody (17356-1-AP)

    WB analysis of mouse kidney using 17356-1-AP (same clone as 17356-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHDH Polyclonal antibody (17356-1-AP)

    IHC staining of human liver cancer using 17356-1-AP (same clone as 17356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17356-1-AP (CHDH antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHDH Polyclonal antibody (17356-1-AP)

    IHC staining of human liver cancer using 17356-1-AP (same clone as 17356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17356-1-AP (CHDH antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17356-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHDH Polyclonal antibody, PBS Only

CHDH Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17356-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17356-1-PBS targets CHDH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10614

Product name: Recombinant human CHDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 244-594 aa of BC034502

Sequence: YLHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKNGQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPITLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAYQVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGSHIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPTIMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR

相同性解析による交差性が予測される生物種
Full Name choline dehydrogenase
Calculated molecular weight 594 aa, 65 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC034502
Gene Symbol CHDH
Gene ID (NCBI) 55349
RRIDAB_2276253
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NE62
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.

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