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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHD9 Polyclonal antibody (13402-1-AP)

    WB analysis using 13402-1-AP (same clone as 13402-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CHD9 Polyclonal antibody (13402-1-AP)

    WB analysis of K-562 using 13402-1-AP (same clone as 13402-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHD9 Polyclonal antibody (13402-1-AP)

    IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHD9 Polyclonal antibody (13402-1-AP)

    IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Hela cells using CHD9 Polyclonal antibody (13402-1-AP)

    IF Staining of Hela using 13402-1-AP (same clone as 13402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Hela cells using CHD9 antibody (13402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHD9 Polyclonal antibody, PBS Only

CHD9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13402-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13402-1-PBS targets CHD9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4245

Product name: Recombinant human CHD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033770

Sequence: MTDPMMDFFDDANLFGETLEGLSDDAFVQPGPVSLVDELNLGAEFEPLHIDSLNHVQGTPTHQKMTDFEQLNQFDSIKFHHVNQSFGSPAEHVLSPHSQFNCSPIHPQNQPNGLFPDVSDGSPMWGHQTATTISNQNGSPFHQQGHSHSMHQNKSFVAHHDFALFQANEQQTQCTSLRSQQNRNNLNPGQNSLSQSKNFMNVSGPHRVNVNHPPQMTNASNSQQSISMQQFSQTSNPSAHFHKCSSHQEGNFNGPSPNMTSCSVSNSQQFSSHYSFSSNHISPNSLLQSSAVLASNHTNQTLSDFTGSNSFSPHRGIKQESTQHILNPNTSLNSNNFQILHSSHPQGNYS

相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 9
Calculated molecular weight 2897 aa, 326 kDa
Observed molecular weight 330 kDa
GenBank accession numberBC033770
Gene Symbol CHD9
Gene ID (NCBI) 80205
RRIDAB_10640425
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3L8U1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).

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