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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHD7 Polyclonal antibody (31919-1-AP)

    WB analysis using 31919-1-AP (same clone as 31919-1-PBS)

    HEK-293T cells were subjected to Tris-Acetate gel system followed by western blot with 31919-1-AP (CHD7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using CHD7 Polyclonal antibody (31919-1-AP)

    IHC staining of rat cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CHD7 Polyclonal antibody (31919-1-AP)

    IHC staining of mouse cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using CHD7 Polyclonal antibody (31919-1-AP)

    IHC staining of mouse cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CHD7 Polyclonal antibody (31919-1-AP)

    IF Staining of HEK-293 using 31919-1-AP (same clone as 31919-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CHD7 antibody (31919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

CHD7 Polyclonal antibody, PBS Only

CHD7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31919-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ATP-dependent helicase CHD7, CHD-7, Chromodomain Helicase DNA Binding Protein 7, Chromodomain-helicase-DNA-binding protein 7, EC:3.6.4.12

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31919-1-PBS targets CHD7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36595

Product name: Recombinant human CHD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-200 aa of BC110818

Sequence: GLEGLGECGYPENPVNPMGQQMPIDQGFASLQPSLHHPSTNQNQTKLTHFDHYNQYEQQKMHLMDQPNRMMSNTPGNGLASPHSQYHTPPVPQVPHGGSGGGQMGVYPGMQNERHGQSFVDSSSMWGPRAVQVPDQIRAPYQQQQPQPQPPQPAPSGPPAQGHPQHMQQMGSYMARGDFSM

相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 7
Observed molecular weight350 kDa
GenBank accession numberBC110818
Gene Symbol CHD7
Gene ID (NCBI) 55636
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9P2D1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent eukaryotic chromatin remodeling enzyme that regulates nucleosome positioning and alters DNA accessibility, and is essential for organ development.CHD7 is a gene known to be associated with CHARGE syndrome, Kallmann syndrome, and hypogonadotropic hypogonadism, where it is associated with CHARGE syndrome is a congenital multiorgan disorder characterized by eye defects, heart defects, posterior nasal atresia, growth retardation, genital anomalies, ear malformations, and deafness. The effects of CHD7 mutations on inner ear development, neuronal differentiation, cardiovascular development, and regulation of bone lipid homeostasis have been studied.

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