Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, Raji cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86077-2-RR targets CHD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain helicase DNA binding protein 3 |
| Calculated molecular weight | 227 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | NM_005852 |
| Gene Symbol | CHD3 |
| Gene ID (NCBI) | 1107 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12873 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHD3 antibody 86077-2-RR | Download protocol |
| IHC protocol for CHD3 antibody 86077-2-RR | Download protocol |
| WB protocol for CHD3 antibody 86077-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |