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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHD3 Polyclonal antibody

CHD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25335-1-AP

Synonyms

ATP dependent helicase CHD3, CHD 3, CHD3, hZFH, Mi 2a, Mi2 ALPHA, ZFH, Zinc finger helicase

Validation Data Gallery

Applications:
  • WB analysis using 25335-1-AP

    WB analysis using 25335-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25335-1-AP

    WB analysis of HEK-293 using 25335-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25335-1-AP (CHD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25335-1-AP

    IHC staining of mouse brain using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of mouse brain using 25335-1-AP

    IHC staining of mouse brain using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human kidney using 25335-1-AP

    IHC staining of human kidney using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human kidney using 25335-1-AP

    IHC staining of human kidney using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human colon using 25335-1-AP

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human colon using 25335-1-AP

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human colon using 25335-1-AP

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human colon using 25335-1-AP

    IHC staining of human colon using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human ovary using 25335-1-AP

    IHC staining of human ovary using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CHD3 antibody included
  • IHC staining of human ovary using 25335-1-AP

    IHC staining of human ovary using 25335-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25335-1-AP (CHD3 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CHD3 antibody included

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inmouse brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHD3 Ready-To-Use IHC Kit. CHD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

25335-1-AP targets CHD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 3
Calculated molecular weight 227 kDa
Observed molecular weight 240 kDa
GenBank accession numberNM_005852
Gene symbol CHD3
Gene ID (NCBI) 1107
RRIDAB_2880033
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHD3, also named as Mi2 ALPHA or Zinc finger helicase, is a 2000 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and two chromo domain. CHD3 localizes in the nucleus and cytoplasm. CHD3 is widely expressed. CHD3 is a component of the histone deacetylase NuRD complex, which participates in the remodeling of chromatin by deacetylating histones. CHD3 is required for anchoring centrosomal pericentric in both interphase and mitosis.

Protocols

Product Specific Protocols
WB protocol for CHD3 antibody 25335-1-APDownload protocol
IHC protocol for CHD3 antibody 25335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols