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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHD2 Polyclonal antibody (21334-1-AP)

    WB analysis using 21334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21334-1-AP (CHD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CHD2 Polyclonal antibody (21334-1-AP)

    WB analysis of HEK-293 using 21334-1-AP

    WB result of CHD2 antibody (21334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHD2 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHD2 Polyclonal antibody (21334-1-AP)

    IHC staining of mouse testis using 21334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CHD2 Polyclonal antibody (21334-1-AP)

    IHC staining of rat brain using 21334-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using CHD2 Polyclonal antibody (21334-1-AP)

    IHC staining of rat brain using 21334-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CHD2 Polyclonal antibody (21334-1-AP)

    IHC staining of mouse testis using 21334-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHD2 Polyclonal antibody (21334-1-AP)

    IF Staining of HeLa using 21334-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CHD2 Polyclonal antibody (21334-1-AP)

    IF Staining of HeLa using 21334-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

CHD2 Polyclonal antibody

CHD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21334-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 2, CHD-2, CHD 2, ATP-dependent helicase CHD2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inrat brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21334-1-AP targets CHD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15429

Product name: Recombinant human CHD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-230 aa of BC020810

Sequence: MMRNKDKSQEEDSSLHSNASSHSASEEASGSDSGSQSESEQGSDPGSGHGSESNSSSESSESQSESESESAGSKSQPVLPEAKEKPASKKERIADVKKMWEEYPDVYGVRRSNRSRQEPSRFNIKEEASSGSESGSPKRRGQRQLKKQEKWKQEPSEDEQEQGTSAESEPEQKKVKARRPVPRRTVPKPRVKKQPKTQRGKRKKQDSSDEDDDDDEVPKRQTRRRAAKNV

相同性解析による交差性が予測される生物種
Full Name chromodomain helicase DNA binding protein 2
Calculated molecular weight 1828 aa, 211 kDa
Observed molecular weight230-250 kDa
GenBank accession numberBC020810
Gene Symbol CHD2
Gene ID (NCBI) 1106
RRIDAB_3085652
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14647
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromodomain helicase DNA binding protein 2 (CHD2), a member of the sucrose nonfermenting (SNF-2) protein family of epigenetic regulators, which use the energy from ATP hydrolysis to change nucleosome positioning, composition and chromatin structure(PMID: 33435571). CHD2 protein plays a critical role in embryonic development, tumor suppression and survival(PMID: 24834135). In addition, CHD2 has been proposed to prevent breast cancer initiation, and CHD2 mutations are associated with chronic lymphocytic leukemia.

Protocols

Product Specific Protocols
IF protocol for CHD2 antibody 21334-1-APDownload protocol
IHC protocol for CHD2 antibody 21334-1-APDownload protocol
WB protocol for CHD2 antibody 21334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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