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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD4 Monoclonal antibody (66718-1-Ig)

    WB analysis using 66718-1-Ig (same clone as 66718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CHCHD4 Monoclonal antibody (66718-1-Ig)

    WB analysis of rat brain using 66718-1-Ig (same clone as 66718-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66718-1-Ig (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CHCHD4 Monoclonal antibody (66718-1-Ig)

    IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CHCHD4 Monoclonal antibody (66718-1-Ig)

    IHC staining of human testis using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66718-1-Ig (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SK-BR-3 cells using CHCHD4 Monoclonal antibody (66718-1-Ig)

    IF Staining of SK-BR-3 using 66718-1-Ig (same clone as 66718-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using CHCHD4 antibody (66718-1-Ig, Clone: 3E4C6 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66718-1-PBS in a different storage buffer formulation.

CHCHD4 Monoclonal antibody, PBS Only

CHCHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66718-1-PBS
Clone No.3E4C6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CHCHD4, FLJ31709, MIA40

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66718-1-PBS targets CHCHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15339

Product name: Recombinant human CHCHD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-142 aa of BC033775

Sequence: MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS

相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 4
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC033775
Gene Symbol CHCHD4
Gene ID (NCBI) 131474
RRIDAB_2882069
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8N4Q1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

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