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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHCHD2 Monoclonal antibody (66302-1-Ig)

    WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHCHD2 Monoclonal antibody (66302-1-Ig)

    WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CHCHD2 Monoclonal antibody (66302-1-Ig)

    WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using CHCHD2 Monoclonal antibody (66302-1-Ig)

    WB analysis of rat liver using 66302-1-Ig (same clone as 66302-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHCHD2 Monoclonal antibody (66302-1-Ig)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CHCHD2 Monoclonal antibody (66302-1-Ig)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CHCHD2 Monoclonal antibody (66302-1-Ig)

    IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (66302-1-Ig, Clone: 2G1G10 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHCHD2 Monoclonal antibody, PBS Only

CHCHD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66302-1-PBS
Clone No.2G1G10

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2G1G10, AAG10, Aging-associated gene 10 protein, C7orf17, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66302-1-PBS targets CHCHD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24219

Product name: Recombinant human CHCHD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-151 aa of BC003079

Sequence: MPRGSRSRTSRMAPPASRAPQMRAAPRPAPVAQPPAAAPPSAVGSSAAAPRQPGLMAQMATTAAGVAVGSAVGHTLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCRLANGLA

相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Calculated molecular weight 151 aa, 16 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC003079
Gene Symbol CHCHD2
Gene ID (NCBI) 51142
RRIDAB_2881685
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6H1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

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