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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHCHD1 Polyclonal antibody

CHCHD1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 11728-1-AP

Synonyms

C10orf34, C2360, CHCHD1, Nuclear protein C2360

Validation Data Gallery

Applications:
  • WB analysis of A431 using 11728-1-AP

    WB analysis of A431 using 11728-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11728-1-AP (CHCHD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 11728-1-AP

    IF Staining of A431 using 11728-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 11728-1-AP (CHCHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11728-1-AP targets CHCHD1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHCHD1 fusion protein Ag2325 相同性解析による交差性が予測される生物種
Full Name coiled-coil-helix-coiled-coil-helix domain containing 1
Calculated molecular weight 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC020852
Gene symbol CHCHD1
Gene ID (NCBI) 118487
RRIDAB_2079769
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHCHD1 antibody 11728-1-APDownload protocol
IF protocol for CHCHD1 antibody 11728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols